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renal proximal tubule cell line hk 2  (ATCC)


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    ATCC renal proximal tubule cell line hk 2
    Renal Proximal Tubule Cell Line Hk 2, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/renal+proximal+tubule+cell+lines/HK-2/pm41957793-155-15-22
    Average 99 stars, based on 4520 article reviews
    renal proximal tubule cell line hk 2 - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosis
    Article Snippet: .. The human renal proximal tubule cell lines (HK-2, ATCC) were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F12 (DMEM/F12) (Thermo Fisher Scientific) containing 5% fetal bovine serum (Thermo Fisher Scientific) and 100 Uml streptomycin–penicillin (WelGENE) at 37 °C in a humidified atmosphere containing 5% CO 2 . .. The 70–80% confluent HK-2 cells were incubated in Opti-MEM (Thermo Fisher Scientific) without streptomycin–penicillin for 2 h and transfected with 25 or 50 nmol/l of small interfering RNA (siRNA) targeting Exoc5 (Bioneer) and scrambled siRNA (SN-1002, Bioneer) using Lipofectamine 3000 (Thermo Fischer Scientific) for 6 h according to the manufacturer’s instructions.

    Article Title: Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosis.
    Article Snippet: .. The human renal proximal tubule cell lines (HK-2, ATCC) were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F12 (DMEM/F12) (Thermo Fisher Scientific) containing 5% fetal bovine serum (Thermo Fisher Scientific) and 100 Uml streptomycin–penicillin (WelGENE) at 37 °C in a humidified atmosphere containing 5% CO2. .. The 70–80% confluent HK-2 cells were incubated in Opti-MEM (Thermo Fisher Scientific) without streptomycin–penicillin for 2 h and transfected with 25 or 50 nmol/l of small interfering RNA (siRNA) targeting Exoc5 (Bioneer) and scrambled siRNA (SN-1002, Bioneer) using Lipofectamine 3000 (Thermo Fischer Scientific) for 6 h according to the manufacturer’s instructions.

    Modification:

    Article Title: Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosis
    Article Snippet: .. The human renal proximal tubule cell lines (HK-2, ATCC) were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F12 (DMEM/F12) (Thermo Fisher Scientific) containing 5% fetal bovine serum (Thermo Fisher Scientific) and 100 Uml streptomycin–penicillin (WelGENE) at 37 °C in a humidified atmosphere containing 5% CO 2 . .. The 70–80% confluent HK-2 cells were incubated in Opti-MEM (Thermo Fisher Scientific) without streptomycin–penicillin for 2 h and transfected with 25 or 50 nmol/l of small interfering RNA (siRNA) targeting Exoc5 (Bioneer) and scrambled siRNA (SN-1002, Bioneer) using Lipofectamine 3000 (Thermo Fischer Scientific) for 6 h according to the manufacturer’s instructions.

    Article Title: Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosis.
    Article Snippet: .. The human renal proximal tubule cell lines (HK-2, ATCC) were cultured in Dulbecco’s modified Eagle medium/nutrient mixture F12 (DMEM/F12) (Thermo Fisher Scientific) containing 5% fetal bovine serum (Thermo Fisher Scientific) and 100 Uml streptomycin–penicillin (WelGENE) at 37 °C in a humidified atmosphere containing 5% CO2. .. The 70–80% confluent HK-2 cells were incubated in Opti-MEM (Thermo Fisher Scientific) without streptomycin–penicillin for 2 h and transfected with 25 or 50 nmol/l of small interfering RNA (siRNA) targeting Exoc5 (Bioneer) and scrambled siRNA (SN-1002, Bioneer) using Lipofectamine 3000 (Thermo Fischer Scientific) for 6 h according to the manufacturer’s instructions.



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    Image Search Results


    Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Journal: Comprehensive Physiology

    Article Title: The cardiac circadian clock regulates rhythms in peripheral tissues via Fibulin 5

    doi: 10.1002/cph4.70147

    Figure Lengend Snippet: Treatment of synchronized kidney and muscle cells with recombinant Fbln5 (10ng/mL) disrupts rhythmic gene expression. A,B) Disrupted expression of Clock and Bmal1 but not C) Per2 in mouse myotube C2C12 cells with Fbln5 treatment. D,E) Disrupted expression of Bmal1 and Per1 but not F) Clock in human renal proximal tubule endothelial cells (RPTEC) with Fbln5. n=3 repetitions with biological triplicate. *p<0.05 Vehicle vs Fbln5. Cells were synchronized with dexamethasone prior to treatment with Fbln5 or vehicle.

    Article Snippet: The human Renal Proximal Tubule Epithelial Cell (RPTEC) immortalized cell line (ATCC, PCS-400–010) was cultured in complete growth medium consisting of the base medium containing DMEM: F12 Medium (ATCC 30–2006) and the RPTEC Growth Kit components (ATCC ACS-4007) consisting of 5 pM triiodo-L-thyronine, 10 ng/mL recombinant human EGF, 3.5 μg/mL ascorbic acid, 5.0 μg/mL human transferrin, 5.0 μg/mL insulin, 25 ng/mL prostaglandin E 1 , 25 ng/mL hydrocortisone, 8.65 ng/mL sodium selenite and 1.2 mg/mL sodium bicarbonate.

    Techniques: Recombinant, Gene Expression, Expressing